mouse monoclonal anti ho1  (Novus Biologicals)

Journal:

Article Title: Heat Stress and Hormetin-Induced Hormesis in Human Cells: Effects on Aging, Wound Healing, Angiogenesis, and Differentiation

doi: 10.2203/dose-response.08-014.Rattan

Figure Lengend Snippet: Western blots showing the effect of curcumin on the induction of the stress response marker heme-oxygenase-1 (HO-1) in mid-passage (less than 50% lifespan completed) human skin fibroblasts ASF-2. The antibody used was a commercially purchased mouse monoclonal antibody anti-HO1 (osa110 from Nordic Biosite, Sweden), diluted 1:5000 in PBS containing 1% bovine serum albumin.

Article Snippet: The antibodies used in Western blots were commercially purchased mouse monoclonal antibody osa110 anti-HO1, and spa800 for Hsp27 (Nordic Biosite, Sweden), diluted 1:5000 in PBS containing 1% bovine serum albumin. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIGURE 7 caption a7 Effect of 20 μM rosmarinic acid (RA) treatment for 50 days on senescence markers in late-passage human skin fibroblasts, ASF-2. (A) Light microscopic pictures of ASF2 cells stained for senescence-specific β-galactosidase (microscopic magnification 20x); (B) The proportion of ASF2 cells showing green autofluorescence in FL1 channel.

Techniques: Western Blot, Marker

Journal:

Article Title: Heat Stress and Hormetin-Induced Hormesis in Human Cells: Effects on Aging, Wound Healing, Angiogenesis, and Differentiation

doi: 10.2203/dose-response.08-014.Rattan

Figure Lengend Snippet: Induction of stress proteins Hsp27 and HO-1 in mid-passage (less than 50% lifespan completed) human skin fibroblasts ASF-2 treated with 20 μM rosmarinic acid (RA) for 72 hr. The antibodies used in Western blots were commercially purchased mouse monoclonal antibody osa110 anti-HO1, and spa800 for Hsp27 (Nordic Biosite, Sweden), diluted 1:5000 in PBS containing 1% bovine serum albumin.

Article Snippet: The antibodies used in Western blots were commercially purchased mouse monoclonal antibody osa110 anti-HO1, and spa800 for Hsp27 (Nordic Biosite, Sweden), diluted 1:5000 in PBS containing 1% bovine serum albumin. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIGURE 7 caption a7 Effect of 20 μM rosmarinic acid (RA) treatment for 50 days on senescence markers in late-passage human skin fibroblasts, ASF-2. (A) Light microscopic pictures of ASF2 cells stained for senescence-specific β-galactosidase (microscopic magnification 20x); (B) The proportion of ASF2 cells showing green autofluorescence in FL1 channel.

Techniques: Western Blot